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CYP93Cs


P450 Function Plant Species
CYP93C1 : isoflavone synthase, IFS soybean
CYP93C2 isoflavone synthase, IFS licorice
CYP93C isoflavone synthase, IFS lotus
CYP93C18 isoflavone synthase, IFS pea


CYP93C1:   isoflavone synthase (IFS), oxidation of 7,4'-dihyroxyflavanone (liquiritigenin) or 5,7,4'-trihydroxyflavanone (naringenin) to daidzein or genistein, respectively, soybean (Glycine max).
Jung W, Yu O, Lau SM, O'Keefe DP, Odell J, Fader G, McGonigle B  (2000)  Identification and expression of isoflavone synthase, the key enzyme for biosynthesis of isoflavones in legumes.  Nature Biotech 18: 208-212.  PubMed

CYP93C2:   2-hydroxyisoflavone synthase (IFS), isoflavonoid synthesis, licorice (Glycyrrhiza echinata).
1)  Akashi T, Aoki T, Ayabe S  (1999)  Cloning and functional expression of a cytochrome P450 cDNA encoding 2-hydroxyisoflavanone synthase involved in biosynthesis of the isoflavonoid skeleton in licorice.  Plant Physiol 121: 821-828.  PubMed
2)  Sawada Y, Kinoshita K, Akashi T, Aoki T, Ayabe SI  (2002)  Key amino acid residues required for aryl migration catalysed by the cytochrome P450 2-hydroxyisoflavanone synthase.  Plant J 31: 555-564.  PubMed
3)  Ayabe S-I, Akashi T, Aoki T  (2002)  Cloning of cDNAs encoding P450s in flavonoid/isoflavonoid pathway from elicited leguminous cell cultures.  Methods Enzymol 357: 360-369.

CYP93C:   2-hydroxyisoflavone synthase (IFS), isoflavonoid synthesis, lotus (Lotus japonicus).
Shimada N, Akashi T, Aoki T, Ayabe S  (2000)  Induction of isoflavonoid pathway in the model legume Lotus japonicus: molecular characterization of enzymes involved in phytoalexin biosynthesis.  Plant Science 160: 37-47.  PubMed

CYP93C18:   isoflavone synthase, pea (Pisum sativum).
Cooper LD, Doss RP, Price R, Peterson K, Oliver JE  (2005)  Application of Bruchin B to pea pods results in the up-regulation of CYP93C18, a putative isoflavone synthase gene, and an increase in the level of pisatin, an isoflavone phytoalexin.  J Exp Bot 56: 1229-1237.  PubMed


Comments? Questions?   email:  joan.krochko@nrc-cnrc.gc.ca   website:  P450s in Plants
Plant Biotechnology Institute, National Research Council, Saskatoon, Saskatchewan   CANADA   S7N0W9
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