P450s in Plants What's New and Interesting Arabidopsis Cytochrome P450s P450s in Other Plants

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CYP85A1:   CYP85A2:   steroid-6-oxidase, brassinosteroid biosynthesis, Arabidopsis.
1)  Shimada Y, Fujioka S, Miyauchi N, Kushiro M, Takatsuto S, Nomura T, Yokota T, Kamiya Y, Bishop GJ, Yoshida S  (2001)  Brassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis.  Plant Physiol 126: 770-779.  PubMed
2)  Bancos S, Nomura T, Sato T, Molnar G, Bishop GJ, Koncz C, Yokota T, Nagy F, Szekeres M  (2002)  Regulation of transcript levels of the Arabidopsis cytochrome P450 genes involved in brassinosteroid biosynthesis.  Plant Physiol 130: 504-513.  PubMed
3)  Shimada Y, Goda H, Nakamura A, Takatsuto S, Fujioka S, Yoshida S  (2003)  Organ-specific expression of brassinosteroid-biosynthetic genes and distribution of endogenous brassinosteroids in Arabidopsis.  Plant Physiol 131: 287-297.  PubMed
4)  Nomura T, Kushiro T, Yokota T, Kamiya Y, Bishop GJ, Yamaguchi S  (2005)  The last reaction producing brassinolide is catalyzed by cytochrome P450s, CYP85A3 in tomato and CYP85A2 in Arabidopsis.  J Biol Chem.  JBC Papers in Press (published on February 14th, 2005 as manuscript M414592200).  PubMed

CYP85:   brassinosteroid biosynthesis, C-6 oxidation of 6-deoxocastasterone to castasterone, tomato (Lycopersicon esculentum).
1)  Bishop GJ, Harrison K, Jones JDG  (1996)  The tomato dwarf gene isolated by heterologous transposon tagging encodes the first member of a new family of cytochrome P450.  Plant Cell 8: 959-969.  PubMed
2)  Bishop GJ, Nomura T, Yokota T, Harrison K, Noguchi T, Fujioka S, Takatsuto S, Jones JDG, Kamiya Y  (1999)  The tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis.  Proc Natl Acad Sci USA 96: 1761-1766.  PubMed