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| P450 | Function | Plant Species |
|---|---|---|
| CYP84A1 : | 5-hydroxylase for coniferaldehyde, coniferyl alcohol and ferulic acid (F5H) | Arabidopsis |
| CYP84A3 | 5-hydroxylase for coniferaldehyde, coniferyl alcohol and ferulic acid (F5H) | sweetgum |
| CYP84 | 5-hydroxylase for coniferaldehyde, coniferyl alcohol and ferulic acid (F5H) | Brassica napus |
CYP84A1: 5-hydroxylase for coniferaldehyde, coniferyl alcohol and ferulic acid (F5H, FAH1), phenylpropanoid pathway, Arabidopsis.
1) Meyer K, Cusumano JC, Somerville C, Chapple CC (1996) Ferulate-5-hydroxylase from Arabidopsis thaliana defines a new family of cytochrome P450-dependent monooxygenases. Proc Natl Acad Sci USA 93: 6869-6874. PubMed
2) Meyer K, Shirley AM, Cusumano JC, Bell-Lelong DA, Chapple C (1998) Lignin monomer composition is determined by the expression of a cytocrome P450-dependent monooxygenase in Arabidopsis. Proc Natl Acad Sci USA 95: 6619-6623. PubMed
3) Humphreys JM, Hemm MR, Chapple C (1999) New routes for lignin biosynthesis defined by biochemical characterization of recombinant ferulate 5-hydroxylase, a multifunctional cytochrome P450-dependent monooxygenase. Proc Natl Acad Sci USA 96: 10045-10050. PubMed
4) Ruegger M, Meyer K, Cusumano JC, Chapple C (1999) Regulation of ferulate-5-hydroxylase expression in Arabidopsis in the context of sinapate ester biosynthesis. Plant Physiol 119: 101-110. PubMed
5) Franke R, McMichael CM, Meyer K, Shirley AM, Cusumano JC, Chapple C (2000) Modified lignin in tobacco and poplar plants over-expressing the Arabidopsis gene encoding ferulate 5-hydroxylase. Plant J 22: 223-234. PubMed
6) Jiang H, Morgan JA (2004) Optimization of an in vivo plant P450 monooxygenase system in Saccharomyces cerevisiae. Biotechnol Bioeng 85: 130-137. PubMed
CYP84A3: coniferyl aldehyde 5-hydroxylase, (this paper shows that coniferyl aldehyde 5-hydroxylation is the primary function of CYP84A P450s rather than ferulate-5-hydroxylation), sweetgum (Liquidambar styraciflua), Accession No. AF139532.
Osakabe K, Tsao CC, Li L, Popko JL, Umezawa T, Carraway DT, Smeltzer RH, Joshi CP, Chiang VL (1999) Coniferyl aldehyde 5-hydroxylation and methylation direct syringyl lignin biosynthesis in angiosperms. Proc Natl Acad Sci USA 96: 8955-8960. PubMed
CYP84: ferulate 5-hydroxylase, (Brassica napus).
Nair RB, Joy RW, Kurylo E, Shi X, Schnaider J, Datla RS, Keller WA, Selvaraj G (2000) Identification of a CYP84 family of cytochrome P450-dependent mono-oxygenase genes in Brassica napus and perturbation of their expression for engineering sinapine reduction in the seeds. Plant Physiol 123: 1623-1634. PubMed