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CYP75As


P450 Function Plant Species
CYP75A1 : flavonoid 3,5'-hydroxylase (F3'5'H) petunia
CYP75A2   eggplant
CYP75A3   petunia
CYP75A4   gentian
CYP75A7 flavonoid 3,5'-hydroxylase (F3'5'H) prairie gentian
CYP75A8 flavonoid 3,5'-hydroxylase (F3'5'H) periwinkle
CYP75A10   Torenia
CYP75A flavonoid 3,5'-hydroxylase (F3'5'H) Campanula
CYP75A flavonoid 3,5'-hydroxylase (F3'5'H) Phalaenopsis
CYP75A flavonoid 3,5'-hydroxylase (F3'5'H) Vinca major


CYP75A1:   flavonoid 3', 5'-hydroxylase, petunia (Petunia hybrida).
1)  Horton TA, Brugliera F, Lester DR, Tanaka Y, Hyland CD, Menting JGT, Lu C-Y, Farcy E, Stevenson TW, Cornish EC  (1993)  Cloning and expression of cytochrome P450 genes controlling flower colour.  Nature 366: 276-279.  PubMed
2)  Shimada Y, Nakano-Shimada R, Ohbayashi M, Okinaka Y, Kiyokawa S, Kikuchi Y  (1999)  Expression of chimeric P450 genes encoding flavonoid-3',5'-hydroxylase in transgenic tobacco and petunia plants.  FEBS Lett 461: 241-245.  PubMed

CYP75A2:   eggplant (Solanum melongena cv. Sinsadoharanasu).
Toguri T, Umemoto N, Kobayashi O, Ohtani T  (1993)  Activation of anthocyanin synthesis genes by white light in eggplant hypocotyl tissues, and identification of an inducible P-450 cDNA.  Plant Mol Biol 23: 933-946.  PubMed

CYP75A3:   petunia (Petunia hybrida).
Holton TA, Brugliera F, Lester DR, Tanaka Y, Hyland CD, Menting JGT, Lu C-Y, Farcy E, Stevenson TW, Cornish EC  (1993)  Cloning and expression of cytochrome P450 genes controlling flower colour.  Nature 366: 276-279.  PubMed

CYP75A4:   gentian (Gentiana triflora).
Tanaka Y, Yonekura K, Fukuchi-Mizutani M, Fukui Y, Fujiwara H, Ashikari T, Kusumi T  (1996)  Molecular and biochemical characterization of three anthocyanin synthetic enzymes from Gentiana triflora.  Plant Cell Physiol 37: 711-716.  PubMed

CYP75A7:   flavonoid 3', 5'-hydroxylase, prairie gentian (Eustoma russellianum).
Shimada Y, Nakano-Shimada R, Ohbayashi M, Okinaka Y, Kiyokawa S, Kikuchi Y  (1999)  Expression of chimeric P450 genes encoding flavonoid-3', 5'-hydroxylase in transgenic tobacco and petunia plants.  FEBS Lett 461: 241-245.  PubMed

CYP75A8:   flavonoid 3', 5'-hydroxylase, periwinkle (Catharanthus roseus).
Kaltenbach M, Schroder G, Schmelzer E, Lutz V, Schroder J  (1999)  Flavonoid hydroxylase from Catharanthus roseus: cDNA heterolous expression, enzyme properties and cell-type specific expression in plants.  Plant J 19: 183-193.  PubMed

CYP75A10:    (Torenia hybrida cv. Summerwave Blue).
Suzuki K, Xue H, Tanaka Y, Fukui Y, Fukuchi-Mizutani M, Murakami Y, Katsumoto Y, Tsuda S, Kusumi T  (2000)  Flower color modifications of Torenia hybrida by cosuppression of anthocyanin biosynthesis genes.  Mol Breed 6: 239-246.  

CYP75A:   flavonoid 3',5'-hydroxylase, (Campanula).
Okinaka Y, Shimada Y, Nakano-Shimada R, Ohbayashi M, Kiyokawa S, Kikuchi Y  (2003)  Selective accumulation of delphinidin derivatives in tobacco using a putative flavonoid 3',5'-hydroxylase cDNA from Campanula medium.  Biosc Biotechnol Biochem 67: 161-165.  PubMed

CYP75A:   flavonoid 3',5'-hydroxylase, (Phalaenopsis).
Su V, Hsu BD  (2003)  Cloning and expression of a putative cytochrome P450 gene that influences the colour of Phalaenopsis flowers.  Biotechnol Lett 25: 1933-1939.  PubMed

CYP75A:   flavonoid 3',5'-hydroxylase, (Vinca major).
Mori S, Kobayashi H, Hoshi Y, Kondo M, Nakano M  (2004)  Heterologous expression of the flavonoid 3',5'-hydroxylase gene of Vinca major alters flower color in transgenic Petunia hybrida.  Plant Cell Rep 22: 415-421.  PubMed


Comments? Questions?   email:  joan.krochko@nrc-cnrc.gc.ca   website:  P450s in Plants
Plant Biotechnology Institute, National Research Council, Saskatoon, Saskatchewan   CANADA   S7N0W9
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